A Type II Metacaspase Interacts with RPS1-K-2 in Soybean and Analysis of the Soybean Metacaspase Gene Family
نویسندگان
چکیده
.........................................................................................................9 Introduction ..................................................................................................10 Materials and Methods .................................................................................13 Plant Materials .................................................................................................... 13 Yeast Two-Hybrid Screen .................................................................................... 14 Generation of RNAi Vectors ................................................................................ 15 Agrobacterium rhizogenes Manipulation ........................................................... 15 A. rhizogenes-Mediated Transformation of Soybean Cotyledons ...................... 16 Infection of A. rhizogenes Transformed Cotyledons with P. sojae ...................... 16 RT-PCR ................................................................................................................ 17 Antibody Production and Western Blot Analysis of Gmmc11 p10 and p20 Subunits ................................................................................................ 18 Western Blot Analysis of Protein Extracts from P. sojae Infected and Uninfected Soybean Tissues ....................................................... 18 Results ..........................................................................................................19 Gmmc11 Is a Type II Metacaspase ..................................................................... 19
منابع مشابه
Two classes of highly similar coiled coil-nucleotide binding-leucine rich repeat genes isolated from the Rps1-k locus encode Phytophthora resistance in soybean.
A series of single genes protect soybean from the root and stem disease caused by the oomycete pathogen Phytophthora sojae. In the last two decades, Rps1-k has been the most stable and widely used Phytophthora resistance gene for the major soybean-producing regions of the United States. Four highly similar genes encoding coiled coil-nucleotide binding-leucine rich repeat (CC-NB-LRR)-type protei...
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